prikaz prve stranice dokumenta Elektroforetska analiza proteina membrana jaja
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undergraduate thesis
Elektroforetska analiza proteina membrana jaja
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2023. urn:nbn:hr:109:736983
Katana, Stela
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Katana, S. (2023). Elektroforetska analiza proteina membrana jaja (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:736983

Katana, Stela. "Elektroforetska analiza proteina membrana jaja." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2023. https://urn.nsk.hr/urn:nbn:hr:109:736983

Katana, Stela. "Elektroforetska analiza proteina membrana jaja." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2023. https://urn.nsk.hr/urn:nbn:hr:109:736983

Katana, S. (2023). 'Elektroforetska analiza proteina membrana jaja', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 05 May 2024, https://urn.nsk.hr/urn:nbn:hr:109:736983

Katana S. Elektroforetska analiza proteina membrana jaja [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2023 [cited 2024 May 05] Available at: https://urn.nsk.hr/urn:nbn:hr:109:736983

S. Katana, "Elektroforetska analiza proteina membrana jaja", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:109:736983

Metadata
TitleElektroforetska analiza proteina membrana jaja
Title (english)Electrophoretic Analysis of Eggshell Membrane Proteins
AuthorStela Katana
MentorIvica Strelec (mentor)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Applied Chemistry and Ecology)
Osijek
Defense date and country2023-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Engineering
Abstract
Cilj ovog završnog rada bio je provesti elektroforetsku analizu proteina (SDS-PAGE) membrana jaja pripravljenih različitim postupcima obrade ljuske jaja kao otpada poljoprivredno-prehrambene industrije. U prvom koraku su membrane jaja ispitane na optimalno vrijeme termičke ekstrakcije pomoću pufera za obradu proteina za SDS-PAGE tijekom 30, 60 i 120 minuta, pri čemu je ustanovljeno da je za potpunu ekstrakciju proteina membrana jaja dostatno vrijeme od 30 minuta termičke obrade. U sljedećem koraku su membrane jaja ispitane na razliku u sastavu/intenzitetu proteinskih vrpci ekstrahiranih iz membrana jaja tijekom 30 minuta. SDS-PAGE analiza je pokazala da se najviše proteinskih vrpci membrana jaja detektira u membranama jaja pripravljenim kiselinskom obradom ljuske jaja sa 10 % octenom i 15 % o-fosfornom kiselinom.
Abstract (english)
The aim of this undergraduate thesis was to perform SDS-PAGE analysis of eggshell membrane (ESM) proteins of ESM’s prepared by different eggshell waste processing procedures. In the first step, eggshell membranes were analysed for the optimal time of thermal extraction of ESM proteins using SDS-PAGE of samples treated by SDS-PAGE treatment buffer for 30, 60 and 120 minutes, where it was found that 30 minutes of thermal treatment is sufficient for complete protein extraction of the eggshell membrane proteins. In the next step, the eggshell membranes were analysed for differences in the composition/intensity of the protein bands extracted from the eggshell membranes during 30-minute treatment with SDS-PAGE treatment buffer. SDS-PAGE analysis showed that the most protein bands of eggshell membranes were detected in eggshell membranes prepared by acid treatment of eggshell waste with 10% acetic and 15% o-phosphoric acid.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:736983
ProjectNumber: IP-2020-02-6878
Title: Imobilizacija lipaza na funkcionalizirane nosače na bazi odabranih otpada iz poljoprivredno-prehrambene industrije
Title: Immobilization of Lipases on Functionalised Carriers Produced from Selected Agro-Food Industrial Waste
Acronym: ImoLipWaste
Leader: Sandra Budžaki
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Food Technology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2023-09-21 08:37:41