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master's thesis
Oksidacija katehola lakazom iz Trametes versicolor u šaržnom reaktoru i mikroreaktoru
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2024. urn:nbn:hr:109:173008
Raguž, Željka
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Thermodynamics and Reaction Engineering

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Raguž, Ž. (2024). Oksidacija katehola lakazom iz Trametes versicolor u šaržnom reaktoru i mikroreaktoru (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:173008

Raguž, Željka. "Oksidacija katehola lakazom iz Trametes versicolor u šaržnom reaktoru i mikroreaktoru." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2024. https://urn.nsk.hr/urn:nbn:hr:109:173008

Raguž, Željka. "Oksidacija katehola lakazom iz Trametes versicolor u šaržnom reaktoru i mikroreaktoru." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2024. https://urn.nsk.hr/urn:nbn:hr:109:173008

Raguž, Ž. (2024). 'Oksidacija katehola lakazom iz Trametes versicolor u šaržnom reaktoru i mikroreaktoru', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 28 April 2024, https://urn.nsk.hr/urn:nbn:hr:109:173008

Raguž Ž. Oksidacija katehola lakazom iz Trametes versicolor u šaržnom reaktoru i mikroreaktoru [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2024 [cited 2024 April 28] Available at: https://urn.nsk.hr/urn:nbn:hr:109:173008

Ž. Raguž, "Oksidacija katehola lakazom iz Trametes versicolor u šaržnom reaktoru i mikroreaktoru", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:109:173008

Metadata
TitleOksidacija katehola lakazom iz Trametes versicolor u šaržnom reaktoru i mikroreaktoru
Title (english)Oxidation of Catechol by Laccase from Trametes versicolor in a Batch Reactor and a Microreactor
AuthorŽeljka Raguž
MentorMarina Tišma (mentor)
MentorAnđela Zeko-Pivač (pomoć pri izradi, neposredni voditelj)
Committee memberMirela Planinić (predsjednik povjerenstva)
Committee memberMarina Tišma (član povjerenstva)
Committee memberAna Bucić-Kojić (član povjerenstva)
Committee memberKristina Mastanjević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Process Engineering)
(Sub-department of Thermodynamics and Reaction Engineering)
Osijek
Defense date and country2024-02-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Engineering
Abstract
Katehol je jednostavan fenol koji se prirodno u okolišu pronalazi u tragovima, a sve češću primjenu nalazi u raznim industrijama. Ukoliko se višak katehola pravilno ne zbrine, zaostaje u industrijskim otpadnim vodama i svojim nakupljanjem predstavlja potencijalnu opasnost za okoliš i ljudsko zdravlje. U bioprocesima kataliziranima lakazom, može se koristiti kao supstrat za proizvodnju polimera. Lakaze su enzimi koje imaju sposobnost katalize oksidacije fenolnih spojeva. U ovom radu istraživana je oksidacija katehola katalizirana komercijalnom lakazom i vlastito proizvedenom lakazom u različitim vrstama reaktora. U svrhu proizvodnje lakaze proveden je uzgoj Trametes versicolor na pivskom tropu, metodom fermentacije na čvrstim nosačima. Istražena je kinetika oksidacije katehola katalizirana lakazom te su procijenjeni kinetički parametri (Michaelisova konstanta \(K_{m}\) i maksimalna reakcijska brzina \(V_{m}\)). Oksidacije katehola komercijalnom lakazom i sirovim ekstraktom lakaze provedene su u kotlastom reaktoru i kotlastom reaktoru s prihranjivanjem (3 ciklusa). Oksidacija katehola u mikroreaktoru Y-oblika provedena je samo s komercijalnom lakazom.
Abstract (english)
Catechol is a simple phenol naturally found in trace amounts in the environment and is increasingly utilized in various industries. If excess catechol is not properly managed, it remains in industrial wastewater, posing a potential threat to the environment and human health through accumulation. In laccase-catalysed bioprocesses, it can be used as a substrate for polymer production. Laccases are enzymes capable of catalysing the oxidation of phenolic compounds. This study investigates the oxidation of catechol catalyzed by commercial laccase and crude laccase extract in different types of reactors. For laccase production, Trametes versicolor was cultivated on spent brewery grain using solid-state fermentation. The kinetics of catechol oxidation catalyzed by laccase were explored, and the kinetic parameters (Michaelis constant \(K_{m}\) and maximum reaction rate \(V_{m}\)) were estimated. Catechol oxidations using commercial laccase and crude laccase extract were conducted in a batch stirred tank reactor and a fed-batch stirred tank reactor (3 cycles). Catechol oxidation in a Y-shaped microreactor was carried out only with commercial laccase.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:173008
ProjectNumber: IPS-2023-02-6614
Title: Valorizacija proizvodnih ostataka iz tvornica ulja bazirana na konceptu biorafinerije
Title: Valorisation of residues from the vegetable oil industry based on a biorefinery approach
Acronym: VALREO
Leader: Marina Tišma
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: Bilateralni slovensko-hrvatski projekti
Study programmeTitle: Process Engineering
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka procesnog inženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka procesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) icon
Created on2024-02-22 11:38:02