Research done on cultivating vegetables are of high importance, because we must improve breeding conditions and include needed procedures to insure economically justified results. Building upon that, this experiment focuses on monitoring and surveying soil moisture regime. The testing was conducted at the Faculty of Agriculture University of Zagreb, through the vegetation season of April to June of 2017. One of the most important factors in cultivating lettuce is the amount of moisture in the ground. An agrotechnical tool known as mulching is used in order to achieve the optimal amount of moisture present in the soil. The purpose of this experiment is to determine the soil moisture regime in two different settings – a mulched soil and an unmulched soil. PE-film is used as a mulch, as well as eco wool and sheep wool. The experiment was set up by implementing random blocks in three repetitions. Samples were taken decadically at depths of 0 to 10 cm and 10 to 20 cm. To determine the moisture in the soil, a gravimetric method was used. The results of the study showed the positive influence of mulching treatments in such a way that the moisture content in the soil is higher than that of non mulching soil. The result of the expriment showed a positive correlation between a mulched soil and the increase of moisture in it, in contrast to an unmulched soil. The variance analysis (ANOVA) of all current moisture levels in both layers of the soil measurements, have shown that the following factors have an immense impact on the current level of mositure in the soil: day after grafting the lettuce, mulch, and their interaction. Based on the average current moisture level of the soil, taking in cosinderation the different mulching treatments, statistically different results were found between all the soil experiments that underwent mulching and the actual bare ground on depth 0-10 cm. The greatest average current mositure level in the soil was found under PE-film (35,18% vol in layer 0-10 cm and 35,34% vol in layer 10-20 cm), and the lowest value was found under the bare soil (31,29% vol in layer 0-10 cm and 31,66% vol in layer 10-20 cm). The values of the current moisture level druing lettuce vegitation have never dropped below the point of plant wilting (23,70% vol), regardless of what mulch was applied. The obtained findings indicate a need for further research with alternative organic mulches in vegetable cultivation, with yearly monitoring of climactic change, as well as moisture regime.