Beeswax (lat.Cera flava) is a natural wax produced and secreted by honey bees (Apis mellifera L.) bees, which they use to build the comb. The biggest consumer of the beeswax is beekeeping industry. Beekeepers use wax mainly for the production of comb foundations which are used in the beekeeping technology in order to enable the bees faster and controlled construction of the comb. Due to increased market demand, insufficient quantities in the market, relatively high price and lack of routine control, beeswax is often the target of adulteration. Therefore, there are more than 15 substances used for beeswax adulteration present nowadays on the market. These substances may be of different origin (plant, animal and mineral), but the most common adulterant on the market are derivatives of petroleum (different types of paraffin). Parafin is acyclic (aliphatic) hydrocarbon which is considered as one of the most common xenobiotics in modern beekeeping. The presence of paraffin in the beeswax has a negative effect on the chemical communication within honey bee colonies and normal physiological development of the brood. Since there is no routine control of beeswax authenticity (comb foundations) on the market and no internationally prescribed analytical methods for its testing, the results of recent research indicated that the paraffin continuously circulate in the production process of comb foundation. Given that the current scientific knowledge does not give an information on the amount of paraffin that remains in the comb constructed on paraffin comb foundations, which is further used as a raw material for making foundations, the aim of this study was to determine the residual amount of paraffin in the samples collected from the honeycomb collected from experimental honey bee colonies after complete construction by the honeybee workers and blending combs. The samples of honeycomb built on paraffin foundation were collected from experimental hives situated at five locations across Croatia. In order to determine overall chemical composition and the amount of residual paraffin, collected honeycomb samples (n=30) were analyzed using FTIR- ATR spectroscopy. Preliminary results showed that average of 57.96% of paraffin remains in comb constructed on paraffin foundation. The amount of the residual paraffin in analysed samples ranged from 47.78 to 68.65%. This study has demonstrated the presence of a serious problem on the beeswax market that indicates on "chronic" circulation and accumulation of paraffin in the comb foundation production process.