The Saskatoon berries are fruit species from North America. The shape is similar to a blueberry, but the nutrition value is better than blueberry. Saskatoon berries are adaptable to our production area and have huge economic potential. Most common method for propagation of Saskatoon berry is vegetative propagation but micropropagation is used as well. The aim of this work was to investigate the effects of agar, cytokinin, PPM, and gibberellic acid on the success of micropropagation of Saskatoon berries. We have been using shoot tips (1-1,5 mm) isolated from axillary buds for culture establishment. Micropropagated shoots of Saskatoon berries were used in further experiments. Agar (Plant agar) as a component of MS medium had a significant effect on the micropropagation of Saskatoon berry. To determine the optimal concentration of growth regulators we used 6- benzylaminopurine (BAP) and Metatopolin (mT) in the concentration of 0.3 and 1 mg/L and medium without hormones (HFM). Average of highest number of shoots (3.1), considering both concentrations of hormones, was at MS with mT. There was not a significant difference in the length of the shoots considering a type of cytokines. In the length of the shoots, there was not significant, but significant longer shoots were on lower concentration (0.3 mg/L) of cytokinins. As the best treatment (cytokinin x concentration) for a number of shoots was MS with 1 mg/L of mT and they produced 4.7 shoots per explant. Gibberellic acid was not a significant factor for the number of shoots, but better results were at 0.2 mg/L of GA3. Also, biocide (PPM) was not significant in this research. Along with shoot proliferation, the greatest problem of micropropagation is in vitro shoot rooting of Saskatoon berry. Percentage of rooting was 28% on MS with 2 mg/L NAA.