Honey production has become more and more questionable lately because of more frequent extreme climate changes. For this reason, it’s necessary to develop production and other bee products. One of the bee products that deserves attention is certainly bee venom. Therefore, it’s necessary to complete the knowledge on the possibilities of producing bee venom by a modern method of electro stimulation. The study was conducted in Daruvar area. The experiment was carried out on a total of 18 honey bee colonies divided into two groups of nine colonies. Three unified subgroups with three beehives of different strength was separated by Liebefeld method. Collection of bee venom from worker bees was carried out by nine electro stimulation devices. The collection process was carried out over three consecutive days to three different strength colonies with a spacing of one week with the collectors placed at the entrance of the hive and into the hive (on frames). The study showed statistically significant differences in bee venom weight between strong (0.193 g), medium strong (0.164 g) and between medium strong and weak (0.111 g) honey bee colonies. Considering the position of the collector, a statistically significant difference in the weight of bee venom was found with the collectors on frames (0.165 g) compared to collector on entrance of the hive (0.148 g). A statistically significant difference was also found in the amount of collected bee venom in the morning (0.173 g) compared to the amount collected in the afternoon (0.131 g), as well as the amount in the early evening (0.166 g) and afternoon hours. There were no statistically significant differences between the morning and the early evening hours in the amount of collected bee venom.