Agricultural sector is the second largest producer of greenhouse gas emissions. Carbon dioxide (CO2), methane (CH4) and nitrogen oxide (N2O) directly affect the greenhouse effect, whereas nitrogen oxides (NO and NO2, commonly known as NOX) from agricultural production have an effect on the formation of ozone (O3) which is also greenhouse gas. Research of nitrogen oxide emissions was conducted in the field experiment in Potok in 2014. using passive samplers and static chambers developed at the Institute for Medical Research and Occupational Health and Faculty of Agriculture in Zagreb. The aim of the study was to determine the suitability of the selected method for N-NO2 concentration measurement, to calculate N-NO2 emission in two fenophases of triticale and to determine potential impact of mineral fertilization on the N-NO2 emission. Research has shown that the used method was suitable for N-NO2 concentration measurement (measured concentrations were above limit of detection). Statistically significant N-NO2 emission differences between two monitored treatments (300 kg N and 0 kg N) were not observed. Measured daily values of N-NO2 emission ranged from 1,66 to 7,18 mg/ha per day, depending on the phenophase and treatment. Average cumulative annual flux was 1,15 g/ha per year and 1,55 g/ha per year respectively, depending on the treatment.