The aim of the research conducted in the area of Sisak ‐ Moslavina County in Jamarica in 2018 was to determine the influence of seed inoculation on soybean agronomic traits. Three varieties of soybean (Lucija, Regale and Ika) were included in the study, and seed treatment with Biofixin S and control without the inoculation. Variety Lucija belongs to maturity group 00‐0, variety Regale is maturity group 0, and variety Ika 0‐I. Variety Lucija achieved the highest seed yield (2,659 t/ha), then variety Regal (2,305 t/ha), and the lowest yield was achieved by variety Ika (2,126 t/ha). There was no statistically significant difference among the soybean varieties in the harvest index. Variety Lucija had the highest plant height and height up to the first pod. The varieties Regale and Ika had significantly higher nodes number on the main stem compared to the variety Lucija. The variety Ika achieved significantly the highest total nodes number and total fertile nodes number per plant (24.1 and 14.6). The varieties did not differ significantly in the pod and seed number per plant. Varieties Regale and Lucija achieved the highest seed weight per plant. Variety Lucija had the largest number of branches per plant (1.9). Seed inoculation did not have a significant effect on the soybean seed yield as well as on yield components.