Sugar beet is an industrial plant that is grown for production of sugar, because of the high concentration saccharide in its thickened roots. Sugar beet is parasitized by over a hundred pests, among them the twenty plant parasitic nematodes. Species Heterodera schachtii Schmidt1871is the most important and most damaging of all nematodes parasites on sugar beet. As a result of its parasitize, root becomes elongated, appears beard growth and a group of diseased plants in certain areas so called “patches”. Sugar beet yield after the sugar beet nematode attack can be reduced up to 60%. The goal of this graduation thesis is to determine the presence of sugar beet nematodes in the soil samples collected in 2013, on the fields of sugar beet at regionthe location of Tovarnik. At the same time, the aim was also to determine the intensity of infection and the population height on the sampled fields in order to recommend appropriate control measures. Sugar beet cyst nematodes were extracted from dried soil samples with flotation method and flotation Spears device. The intensity of infection and height of the population was determined by the numerous of population of cysts and the vitality was determinated by counting viable eggs and larvae. The presence of sugar beet nematode was found in all of five analysed soil samples, while the average number of cysts was 23 cysts/100 ml soil. The average vitality of cyst was 4.96 eggs and larvae/ g soil, which exceeded economic threshold. Because of the relatively high infestation of this pest on the sampled field, it is necessary to implement at least a six-year crop rotation, weedhost control and sowing oftolerant sugar beet varieties.