Within the program of revitalization autochthonous varieties of Hrvatsko Zagorje (2006 – 2020)., some varieties were discovered which have been considered lost. In this project it was determined that one of the group of varieties known as ‘Beline’ and within them, one stood out, ‘Belina starohrvatska’. The oldest vine from this variety was found near the birthplace of Ljudevit Gaj in city of Krapina. In order to put this old and neglected variety back in modern viticultural production research on suitable sites, and growing practices are required. In the area of Hrvatsko Zagorje at the site of Komor Začretski the experimental vineyard was established for the evaluation of autochthonous varieties including ‘Belina starohrvatska’. Due to the poor fruit set in 2017., shoot topping and defoliation was performed and in next year defoliation only to evaluate its effects on yield, fruit quality and occurrence of Botrytis. In 2017, different variations of experiments were set up: defoliation before flowering with and without topping, topping without defoliation, topping with defoliation after flowering, and control. On the vines which have been defoliated there were less damage of grey mold and sour rot then in the control. Because of the damages yield in the control variation was reduced in 2017 year while all the others had higher yield and concentrations of sugars. Furthermore, total acidity of the must was lower than in the control. Since there was more precipitation in 2017, then in 2018., yield was higher but with lower quality followed with less concentration of the sugars. Topping has showed not to be necessary in 2017. because it did not affect fruit set, so it was not performed in 2018. Even though we cannot influence the weather conditions in a specific year, defoliation showed to be a successful in fight against Botrytis and that it doesn’t reduce grape yield and quality of the must.