Nitrogen deficiency has negative affects on photosynthetic pigments synthesis and photosynthetic dark and light reactions. The aim of this study was to analyse the impact of nitrogen deficiency stress on chlorophyll fluorescence and gas exchange (Calvin cycle) on three bean genotypes. Three common bean genotypes were used: Trešnjevac, Zelenčec and Biser. Treatments were represented as irrigated with ½ Hoagland's solution (N+) and pots irrigated with ½ Hoagland's solution without nitrogen (N-). Chlorophyll content, gas exchange and chlorophyll fluorescence were measured once a week during a period of four weeks. Results have shown that there are significant differences in chlorophyll content index (CCl), maximal photochemical efficiency (Fv/Fm) and net photosynthetic rate (A) among investigated bean genotypes. Nitrogen deficiency had a significant negative impact on CCl, A, stomatal conductance, Fv/Fm, but those parameters (A and Fv/Fm) were least affected in genotype Biser, and this genotype we can consider the most tolerant on nitrogen deficiency stress. These results suggest that nitrogen deficiency had stronger negative effect on chlorophyll content, activity of RuBisCo and other Clavin cycle enzymes compared to photochemical reactions, which is probably the result of nitrogen deficiency on reduced synthesis of photosynthetic proteins include in Calvin cycle.