Stinging nettle (Urtica dioica L.) is a perennial plant species of scientific interest because of its medicinal and nutritional composition. The beneficial medicinal composition is based on biologically active compounds, while the nutritional value is based on the presence of vitamins and minerals, a low content of proteins, fats and a high water content. Wild nettle is a variable substance with unexplored composition, which is why recent research points to the importance of its cultivation. Modern hydroponic cultivation techniques such as floating hydropon, in a greenhouse can eliminate the cultivation problems in the open field. They allow availability of nettle for a longer period of the year, higher yields and better quality of the herb. Optimum conditions for plant growth and shortening of production cycles are achieved by proper management of abiotic factors of air and nutrient solution during cultivation. The objective of this study was to determine the content of vitamin C, total phenols, crude proteins and antioxidant capacity, yield components and yield of fresh nettle herb grown in two different solutions (Jensen and Cooper) and over two harvest periods (1st and 2nd harvest period) using floating hydropon technique. From the research results achieved, it could be concluded that the Jensen solution, whose composition is lower in nutrients, had a better effect on morphological properties while the Cooper solution had a better effect on chemical properties of stinging nettle. The combination of both solutions and the 2nd harvest period resulted in a higher yield of nettles compared to the first harvest period.