In this work, the influence of cytokinins on rooting success of Gala cultivar apple in dependence on pretreatment with cytokinins was studied. The use of cytokinins is necessary in the shoot multiplication phase. However, cytokinin residues may negatively affect shoot rooting. As it is known from the available literature that the natural cytokinin meta-topoline (mT) is degraded faster in plant tissues compared to the more commonly used 6-benzylaminopurine (BAP) and will have fewer negative effects on root development and rooting this study proved the opposite. One of the main reasons for the large application of BAP in micropropagation is that it is more stable compared to other cytokinins, it oxidizes more difficult with the help of light and it is cheaper and easier to obtain. Due to various side effects during use such as shoot tip necrosis, rooting inhibition and difficulty in acclimatization, meta-topoline is cited as an alternative in the literature. In n this study to detect post-effect of cytokinins on rooting of Gala variety apple, shoots of Gala apples were first multiplied on medium with two different growth regulators. Cytokines BAP and mT were used at five different concentrations. After a sufficient number of explants were obtained on the nutrient medium for multiplication, apple shoots were placed on the rooting medium with auxin Indole-3-butyric acid (IBA) and on the control medium. The shoots were rooted on MS medium and the rooting success was evaluated with respect to the previous medium where different concentrations of BAP and mT were used. Shoots that developed on media with addition of mT were much longer compared to shoots developed on media with BAP. However, higher number of multiplied shoots were developed on media containing BAP. Rooting as well as success of acclimatization were more successful in plants developed on media with the addition of BAP.