Potato (Solanum tuberosum L.) is crop that propagate vegetatively, making them susceptible to various diseases, especially viruses transmitted through infected planting material. In order to ensure a good yield and achieve the full potential of varieties, it is important to produce healthy, uninfected planting material. This can be achieved by in vitro methods of virus elimination. The recovered plants must be multiplied, which is possible through micropropagation and/or microtuberization. Microtubers represent the first category of seed potatoes from which the following category is derived, next stage is breeding seed. The aim of this study was to investigate the success of microtuberization of three potato genotypes depending on the type of explants used and the activated charcoal content. Three potato varieties, Desiree, traditional variety Brinjak and Peruvian blue potato (S. tuberosum L.var.violette) were used in this experiment. Plant material was previously propagated on Murashige and Skog (1962) medium without growth regulators with the addition of 2% sucrose and 7g/l plant agar to a sufficient number of shoots for experiment. In the first experiment explants with leaves were used, while in the second experiment leaves were removed. The following types of explants were used to set the experiment: shoot tips, two node segments placed horizontally or vertically and one-node segments placed in placed in medium vertically. Based on the results obtained, it can me concluded that the best quality of microtubers (the larger mass and diameter) was obtained from two-node segments with no leaves removed, on a medium with addition of activated charcoal. Deviation from this rule was recorded only for the percentage of shoots that formed microtubers that was significantly higher when leaf free explants were used. Among the varieties, there was significant difference in number, as well as in weight and diametar of microtubers. A larger number of microtubers per shoot was formed by the Brinjak variety, but they had lower weight and diameter than the microtubers formed by Desiree and Peruvian blue potato.