Stinging nettle (Urtica dioica L.) is a perennial species of the nettle family (Urticaceae). It has been used for centuries as a functional food and is also used in medicine, pharmacy, cosmetics, and the textile industry. Stinging nettle is a plant species with a rich and complex chemical composition, which is why it is still the subject of various research. The aim of this study was to determine the content of bioactive compounds and to determine the nutritional potential of alcoholic extracts from dried nettle leaves with respect to the method and duration of ultrasound-assisted extraction. Alcoholic extracts were prepared from dried nettle leaf powder (2 g) using 50% ethyl alcohol (150 mL) as solvent. The prepared solutions were treated with high intensity ultrasound, varying the type of device (probe and bath) and the duration of treatment (5, 10, 15 and 30 min). The following physico-chemical parameters of the solutions were determined by the study: density, electrical conductivity, total acids content, pH and color; while the content of specialized metabolites is determined by: vitamin C, total phenols (flavonoids and non-flavonoids), total chlorophylls, total carotenoids and antioxidant capacity. The highest values of vitamin C, phenolics, non-flavonoids, total chlorophylls and carotenoids, and antioxidant capacity were determined in the sample treated with ultrasonic probe for a period of 15 minutes. It can be concluded that ultrasonic probe treatment is extremely effective in isolating bioactive compounds in a very short time. Moreover, the analysis of the alcoholic extracts of nettle can show that it is a nutritionally valuable product with many potential functional properties.