Due to the numerous positive effects on plant growth and development, numerous biostimulators are used in agriculture today. They are preparations that contain substances and/ or organisms whose role is to stimulate natural processes in plants. The aim of this study was to determine the effect of foliar calcium materials on total acids, pH and the amount of sugar in pepper fruit. The field fertilization experiment was carried out ate the experimental field of Maksimir of the Department of Seed Science of the Faculty of Agriculture, University of Zagreb on two cultivars of pepper (Capsicum annum L.) ‘Šorokšari’ and ‘Kurtovska kapija’. Pepper seedlings were grown in a greenhouse and transplanted to the field at a row spacing of 70 cm and 40 cm spacing within the rows. The experiment included 4 foliar fertilization treatments with calcium growth enhancers. Calcium growth enhancers were applied foliarly 3 time in vegetation. Two fruit harvests were performed. It was found that the use of biostimulants did not have a significant effect on the content of total acids, while an increase in the content of sugar, and pH was confirmed. The highest value of the amount of sugar was achieved in the variety ‘Kurtovska kapija’ using high concentration of Ecogreen in the second sampling and is 10.1 %, the highest value of total acids was achieved in the variety ‘Kurtovska kapija’ using Ecogreen and was 3.66 g/L, while the highest pH value was achieved in variety ‘Šorokšari’ using Zeogreen in the first sampling and was 5.85.