Stinging nettle (Urtica dioica L.) is a wild species that grows in neglected and humus-rich soils, but its cultivation can be planned in a greenhouse using modern hydroponic cultivation techniques. The aim of this study was to determine the content of vitamin C and pigment compounds (chlorophyll a, chlorophyll b, total chlorophyll and carotenoids) of nettle leaves depending on the composition of the nutrient solution in floating hydroponics. Vitamin C is known as a potent antioxidant, and chlorophylls and carotenoids, which are important for primary metabolism, are significantly affected by biotic, abiotic and anthropogenic factors, and the choice of cultivation method can significantly influence their content in the raw material. The monofactorial experiment was laid out according to the randomized blockchain method in three replicates in a floating hydropon, and the key factor was the composition of the nutrient solution. Two types of nutrient solutions were varied according to the recipe of Johnson (lower nitrogen content) and Larson (higher nitrogen content). After statistical data processing, no significant effect of the solution was found on the content of all the mentioned bioactive compounds analyzed. However, a slightly higher content of vitamin C (120 mg/100 g of fresh weight) was found in fresh leaves of nettle grown in nutrient solution according to the Larson recipe, while a lower content of pigment compounds was found in fresh leaves of nettle grown in nutrient solution according to Johnson recipe (total chlorophyll 0.85 mg / 100 g fresh matter, carotenoids 0.19 mg/100 g fresh weight). Based on the conducted analysis, samples of fresh nettle leaves grown in floating hydropon had high levels of vitamin C and pigment compounds in the first mowing period, regardless of the composition of the nutrient solution. Based on the all mentioned, can be concluded hatfresh nettle leaves can be considered a nutritionally valuable raw material.