The effect of climate change has a great influence on the development of the phenophases of the grapevine, and consequently on the quality and yield of the grapes. Recently, new methods have been researched that could help grapes to achieve a satisfactory result, despite climate changes. One of such interventions is late pruning, which is performed later than usual. The aim of this work was to demonstrate the effect of late pruning on the development cycle of the grapevine, yield and chemical composition of grapevien variety ˈPortugizacˈ, which was chosen as an experimental variety due to its early ripening. The research was conducted in 2021 according to a random block arrangement with three pruning treatments: standard winter pruning (control), pruning at the moment of budbreak and pruning at the moment of appearance of 2-3 separate leaves. ˈ Through the conducted research on the influence of late pruning on the chemical composition of grapes ˈPortugizacˈ, it was concluded that late pruning, which was performed at the time of budburst, resulted in a decrease in the content of sugar, but also in total acids, and in an increase in total polyphenols, anthocyanins, flavonols, stilbenes and phenolic acids in the grape skin compared to the standard winter pruning. Late pruning performed at the time of opening of 2-3 leaves resulted in a decrease in sugar content, but also in total polyphenols and anthocyanins, and in an increase in the concentration of total acidity, total flavanols, stilbene and phenolic acids.