The flora of Brijuni National Park has not been systematically researched for years, and the last such research was conducted in 1908 by Makowsky. Recently, the flora of Mali Brijun was investigated by Bogdanović and Ljubičić in 2018, while the flora of Veli Brijun and 12 neighbouring islands and islets remained completely unexplored. Field research was conducted during 2020-2021 in order to floristically investigate and mapping the flora of the entire NP Brijuni, and a total of 759 taxa were identified, of them 202 for the first time. There are a total of 102 recorded families, of which the most represented are grasses (Poaceae) with 86 taxa and legumes (Fabaceae) with 85 taxa. Out of the total number, 30 species are endangered. Regarding the life forms in the flora of NP Brijuni, the most numerous are therophytes with 307 taxa, hemicryptophytes with 233 taxa, phanerophytes with 78 taxa, geophytes with 71 taxa, hamephytes with 58 taxa and hydrophytes with 11 taxa. There are 14 invasive species, and 74 species of cultivated plants. Only one endemic species (Limonium cancellatum) was recorded.