In the Republic of Croatia, a high percentage of infected vineyards of autochthonous grapevine varieties has been recorded, with virus infections representing the biggest problem. Using in vitro methods, the process of freeing the vine from the virus is carried out. With this research, the aim was to determine the impact of the sanitary status of clones of varieties "Plavac mali" and "Pošip" on the growth and development of explants. Clones of the varieties "Plavac mali" and "Pošip" with different sanitary statuses in terms of virus infections (GVA, GFLV, GLRaV-1, GLRaV-1+GLRaV-3) were introduced into tissue culture using different categories of buds from the initial shoots (a - apical meristem, b - 2nd and 3rd bud on the initial shoot, and c - 4th and 5th bud on the initial shoot), and they were inoculated onto a medium (MS+0.5 mg/L BAP + 0.05 mg/l IAA) using the meristem culture method. The growth and development of explants in tissue culture were measured after four and eight weeks of cultivation. The results show a significant impact of the variety on the in vitro growth and development of explants, while the sanitary status of clones within the varieties and the categories of buds did not significantly affect the measured parameters of height, number of nodes, and the occurrence of calluses, except that the bud category significantly influenced the formation of callus tissue. Further research is needed to complement the obtained results regarding the tested varieties.