Strawberries are a popular fruit their production is continuously increasing. Strawberries are well known for their attractive appearance, and also for their pleasant aroma and taste. An ever-increasing number of research papers is confirming the positive influence of strawberry consumption on human health. The chemical composition of strawberry fruit is probably responsible for this. However, with strawberries currently present on the market, most attention is given to the size, shape and color of the fruits, while a lot less attention is given to their chemical composition. Therefore, the goal of this research was to analyze the fruits of woodland strawberry and garden strawberry and determine the difference between their chemical composition. The fruits of garden strawberry (Fragaria x ananassa), cultivar 'Sonata' and three different populations of woodland strawberry (Fragaria vesca) were used in this analysis. The fruits were analyzed for total soluble solids content, total acidity, concentration of total flavonoids, total phenols, and total anthocyanins. It was determined that the fruits of woodland strawberry had a higher amount of total soluble solids content, higher concentration of total flavonoids and higher concentration of total phenols. Total acidity was approximately the same for all fruits. Only the concentration of total anthocyanins was higher in the fruits of garden strawberry.