Endotherapeutic methods of pesticide application provide more efficient use of chemicals, reduced negative impact on the environment, and the possibility of application in situations where soil or foliar spraying is difficult to perform. The method of direct injection of pesticides enables targeted action of the pesticide and better distribution within the plant structure. The advantages of endotherapy include selective action, long-lasting plant protection, and preservation of ecosystems. Additionally, endotherapy offers a sustainable approach to plant protection and reduced pesticide use. The application of endotherapy in disease and pest control in agriculture has many benefits, including targeted application, protection in windy conditions, no plant damage, reduced risk of leaching, and lower amounts of plant protection products. However, it may have drawbacks such as slower absorption, challenges on public surfaces, the need for appropriate equipment and expertise. The application of endotherapy in the treatment of plant diseases such as powdery mildew of grapevine, downy mildew of grapevine, fungal canker of walnut, brown leaf spot of chestnut, apple scrab and olive quick decline syndrome shows promising results in suppressing these diseases.