Sugar beet production in Croatia is an economically significant economic activity whose production can be threatened by various pests. The beet flea beetle and sugar beet weevil are the most important pests of sugar beet in the early stages of its development. If they appear in large numbers, they can completely destroy the crop already at the beginning of the growing season. Due to the cancellation of the license for the use of neonicotinoids for the treatment of all agricultural crops, including sugar beets, producers are forced to use other, alternative methods to protect the crop. One of the potential methods of seed protection is resistant genotypes and seed treatment with more environmentally friendly insecticides. The research was conducted in the laboratory of the Institute of Agricultural Zoology with the adult form of the beet flea beetle and sugar beet weevilcollected in the Požega-Slavonia County. During the research, the effectiveness of seed treatment with different insecticides and their combinations (classical and biological treatment) was determined, with a total of 18 seed varieties. The research results show that genotype 3 (variant with slow germination) shows less damage than the other two genotypes. Also, alternative methods such as the use of environmentally acceptable insecticides azadirachtin, flupyradifuron and pyrethyrin give satisfactory results in protecting sugar beet from pests during the critical phase of development (BBCH 12). This research highlights the importance of introducing new active ingredients, of a positive ecotoxicological nature, with the aim of protecting sugar beet and increasing high-quality yields while preserving the ecosystem and human health.