Fusarium blight (FHB), caused by the fungus Fusarium graminearum (Gibberella zeaeteleomorph (Schw.) Petch) is currently one of the most dangerous diseases of wheat in theworld. The damage caused by the infection manifests in the reduction of grain yield and accumulation of mycotoxins harmful for human and animal health. The development of resistant cultivars is considered to be the most effective method of disease control. In breeding for resistance to FHB different methods of artificial ear inoculation have been used including the most common method of spraying of inoculum on wheat ears in the flowering stage (spraymethod). This method is technically and labor demanding and as an alternative a surface scattering of infected maize stalks on the soil surface after the emergence of wheat have also been proposed (maize stalk method). When choosing an appropriate method of artificial inoculation in addition to cost and time required for its implementation, a high heritability of the method is required to obtain reliable and reproducible results. The aim of the study was, in a field experiment including 18 breeding lines from Bc Institute Zagreb and 5 checks with different levels of FHB resistance, to compare the heritability of two methods of artificial inoculation (spray and maize stalk methods) and a method of natural infection with fungi that causes FHB of wheat and to assess the phenotypic correlation between these methods. Significant differences among genotypes was determined for Visual Rating Index (VRI) for all three methods of infection while for the percent Fusarium damaged kernels (FDK) differences were significant only for two methods of artificial inoculation. For both ratings VRI and FDKa significant interaction between method and genotype was determined. The level of resistance to FHB in several breeding lines was similar to the resistant standards. For VRI a strong positive correlation (0.85 to 0.91) between all three methods was found, whereas in the case of FDKstrong correlation was observed only between the two methods of artificial inoculation (0.86). For all three methods heritability was significantly higher for VRI (0.59 to 0.95) than for FDK(0.04 to 0.54). Also, the heritability for both SAI and FDK was significantly higher for the two methods of artificial inoculation than for natural infection.