The role of the aurochs (B. primigenius) during domestication and it's impact on the formation of modern cattle breeds (B. taurus) is not entirely clarified. There are two oposite theories. According to first one, all modern cattle breeds have a unique origin from the Middle East and were created by domestication of the wild local population of aurochs. According to the other, modern cattle breeds have multiple origin with genetic contribution of European aurochsen and gene flow from North Africa to Southern Europe. The aim of this paper is to resconstruct the variability distribution of mtDNA from modern and ancient cattle trough space and time to further clarify the impact of aurochs on the formation of modern breeds. The analysis included total 635 sequences of mtDNA control region, 237 bp long, from modern and ancient samples from the NCBI database and own resources. Analysis of sequences was performed using MEGA7, DNasp5 and Mitotoolpy. Vizualization of variability distribution and phylogenetic relationships was performed via MJ network in PopArt. The results showed high variability of mtDNA in cattle and increased variability over time. The analysis of haplogroups revealed the existence of all major haplogroups in the selected samples set. The unknown haplogroups of ancient samples were determined. The influence of North African genes on the formation of South European cattle breeds was confirmed. By analyzing geographical distribution, at least three possible locations of independent domestication were identified. From these locations domesticated cattle could migrate to the other locations and durig the migration the introgression of local aurochsen could occur. For more precise and accurate determination of the phylogeny of modern cattle breeds and revealing domestiction events, it is necessary to include other regions of mtDNA. Different influences (eg. selection) on each region should also be included. The influence of molecular clock and radiometrical dating of ancient samples should be performed in anaysis of samples with large time span. Also the use of available new technologies would be recommended.