Common Bean (Phaseolus vulgaris L.) is the most important grain legume for human consumption worldwide. In Croatia, common bean is a neglected crop and it is cultivated by small-scale farmers in a traditional way. One of the most widely used landraces in production is 'Trešnjevac'. Croatian landraces are adapted to specific growing conditions, showing a great variability in morphological and agronomic traits. 106 accessions of common bean landraces 'Trešnjevac' were analysed using 19 microsatellite markers (SSR), from which 78 accessions were bush common bean landraces 'Niski Trešnjevac' and 28 accessions were climbing common bean landraces 'Visoki Trešnjevac'. A total of 65 different alleles were detected, ranging from 2 to 8 alleles per microsatellite locus with an average of 3,824. Number of alleles per locus within 'Niski Trešnjevac' was ranging from 1 to 6 with an average of 2.706 per locus; and within 'Visoki Trešnjevac' from 2 to 5 with an average of 3.059 per locus. Allelic richness (Nar) within 'Niski Trešnjevac' was ranging from 1.000 to 4.666 with an average of 2.353 per locus, and within 'Visoki Trešnjevac' from 1.741 to 4.563 with an average of 2.979 per locus. Out of 17 loci, 5 was monomorphic within 'Niski Trešnjevac', and within 'Visoki Trešnjevac' they were all polymorphic. Expected heterozygosity (HE) for both groups was very low as expected due to the fact that bean was a self- pollinated crop. Out of 65 alleles, 13 were identified within 'Niski Trešnjevac', and 19 within 'Visoki Trešnjevac', and 33 alleles was identified in both groups.