The aim of the paper was to determine the subjective assessment of students, alumni and university professors of the Department of Biology at the J. J. Strossmayer University in Osijek on the development of skills that make certain key competences during and after the study and determine their assessment of the importance of key competences in terms of work and career. This paper evaluated to what extent the individual study programs within the Department of Biology are planned to develop key competencies. The point of view of university professors on their need for professional development of teaching competencies, their habits and ways of professional development have also been established. For this purpose, during the academic year 2018/2019, closed type surveys and self-evaluation forms were administered and the respective programs of the Department of Biology were analyzed. The development of some key competencies is planned depending on the study program, whereas the curriculum does not plan the development of Learning Competence, Social and Civic Competence and Cultural Sensitization and Expression. Most of the students' skills are scarcely developed during the studies. However, the students, same as alumni, think that they are important for their work. The largest number of professors cite the organization of workshops and seminars as a resource needed for the development of teaching competencies and claim that their own development of teaching competences is accompanied by feedback from students. There is a disparity between university professors and students when it comes to evaluating the representation of different habits and teaching methods.