Antimicrobial resistance is a growing problem today. An increasingly frequent defense mechanism of bacteria is the expulsion of harmful substances using membrane proteins, i.e. efflux using MDR ("Multiple Drug Resistance") pumps. MDR efflux pumps can extrude majority of clinically relevant antibiotics from cell to environment. Resistant bacteria that express these pumps are ubiquitous in the environment, including soils. Different microorganisms found in the soil form biofilms that help them defend against antibiotics, and MDR pumps help them in the formation itself. To date, many studies have been carried out on the topic of bacterial efflux, but the efflux of mixed biofilms from the soil has remained almost unexplored, and the methods limited. In this research, new methods of cultivation and formation of biofilms from soil samples were established. New methods of measuring efflux using FDA and rhodamine 6G were developed. Also, efflux inhibitors were used during efflux research; verapamil and carbonyl cyanide m-chlorophenyl hydrazone. Wet and dried samples of 8 soils of different composition and origin were used in the research, and their efflux abilities were compared with each other.