prikaz prve stranice dokumenta Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells
  Preuzmi
PDF 1.65 MB
Znanstveni rad - Izvorni znanstveni rad
Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells
Biochimica et Biophysica Acta (BBA) - General Subjects, 1867 (2022), 2; 130283. https://doi.org/10.1016/j.bbagen.2022.130283
Mourya, Atul; Pingle, Purva; Babu, Chanti Katta; Veerabomma, Harithasree; Sainaga Jyothi, Vaskuri G.S; Novak, Jurica; Pathak, Prateek; Grishina, Maria; Verma, Amita; Kumar, Rahul; Singh, Pankaj Kumar; Khatri, Dharmendra Kumar; Singh, Shashi Bala; Madan, Jitender

Institucijski repozitorij: Repozitorij Fakulteta biotehnologije i razvoja lijekova

Citirajte ovaj rad

Mourya, A., Pingle, P., Babu, C. K., Veerabomma, H., Sainaga Jyothi, V. G., Novak, J. ... Madan, J. (2022). Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells. Biochimica et Biophysica Acta (BBA) - General Subjects, 1867. (2). doi: 10.1016/j.bbagen.2022.130283

Mourya, Atul, et al. "Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells." Biochimica et Biophysica Acta (BBA) - General Subjects, vol. 1867, br. 2, 2022. https://doi.org/10.1016/j.bbagen.2022.130283

Mourya, Atul, Purva Pingle, Chanti Katta Babu, Harithasree Veerabomma, Vaskuri G.S Sainaga Jyothi, Jurica Novak, Prateek Pathak, et al. "Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells." Biochimica et Biophysica Acta (BBA) - General Subjects 1867, br. 2 (2022). https://doi.org/10.1016/j.bbagen.2022.130283

Mourya, A., et al. (2022) 'Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells', Biochimica et Biophysica Acta (BBA) - General Subjects, 1867(2). doi: 10.1016/j.bbagen.2022.130283

Mourya A, Pingle P, Babu CK, Veerabomma H, Sainaga Jyothi VG, Novak J, i sur.. Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells. Biochimica et Biophysica Acta (BBA) - General Subjects [Internet]. 19.11.2022. [pristupljeno 15.05.2024.];1867(2). doi: 10.1016/j.bbagen.2022.130283

A. Mourya, et al., "Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells", Biochimica et Biophysica Acta (BBA) - General Subjects, vol. 1867, br. 2, Studeni 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:193:590974. [Citirano: 15.05.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:193:590974
Podaci o radu
Naslov (engleski)Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells
AutorAtul Mourya
AutorPurva Pingle
AutorChanti Katta Babu
AutorHarithasree Veerabomma
AutorVaskuri G.S Sainaga Jyothi
AutorJurica Novak
AutorPrateek Pathak https://orcid.org/0000-0002-6160-9755
AutorMaria Grishina
AutorAmita Verma
AutorRahul Kumar
AutorPankaj Kumar Singh
AutorDharmendra Kumar Khatri
AutorShashi Bala Singh
AutorJitender Madan
Autorova ustanovaSveučilište u Rijeci
(Fakultet biotehnologije i razvoja lijekova)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Kemija
Primijenjena kemija
Sažetak (engleski)
Background: Neuroblastoma is one of the most common malignancies in childhood, accounts for approximately 7% of all malignancies. Andrographolide (AN) inhibits cancer cells progression via multiple pathways like cell cycle arrest, mitochondrial apoptosis, NF-κβ inhibition, and antiangiogenesis mechanism. Despite multiple advantages, application of AN is very limited due to its low aqueous solubility (6.39 ±0.47 μg/mL), high lipophilicity (log P ~ 2.632 ±0.135), and reduced stability owing to pH sensitive lactone ring. Objectives and results: In present investigation, a molecular complex of AN with soya-L-α-phosphatidyl choline (SPC) was synthesized as ANSPC and characterized by FT-IR and1H NMR spectroscopy. Spectral and molecular simulation techniques confirmed the intermolecular interactions between the 14-OH group of AN and the N+(CH3)3 part of SPC. In addition, molecular dynamics (MD) simulation was used to determine the degree of interaction between various proteins such as TNF-α, caspase-3, and Bcl-2. Later, ANSPC complex was transformed in to self-assembled soft nanoparticles of size 201.8 ±1.48 nm with PDI of 0.092 ± 0.004 and zeta potential of 21.7 ± 0.85 mV. The IC50 of free AN (8.319 μg/mL) and the self-assembled soft ANSPC nano-particles (3.406 μg/mL ~ 1.2 μg of AN) against Neuro2a cells was estimated with significant (P <0.05) difference. Interestingly, the self-assembled soft ANSPC nanoparticles showed better endocytosis compared to free AN in Neuro2a cells. In vitro biological assays confirmed that self-assembled soft ANSPC nanoparticles induces apoptosis in Neuro2a cells by declining the MMP (Δψm) and increasing the ROS generation. Conclusion: Self-assembled soft ANSPC nanoparticles warrant further in-depth antitumor study in xenograft model of neuroblastoma to establish the anticancer potential.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaBiochimica et Biophysica Acta (BBA) - General Subjects
Brojčani podacivol. 1867, br. 2, 130283
p-ISSN0304-4165
DOIhttps://doi.org/10.1016/j.bbagen.2022.130283
URN:NBNurn:nbn:hr:193:590974
Datum objave elektroničkog izdanja2022-11-19
URL dokumentahttps://www.sciencedirect.com/science/article/pii/S030441652200201X
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Datum isteka embarga: 2024-01-01
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2022-11-30 13:21:12