The topic of this paper is the influence of store layout on customer behavior. The paper studies the key elements of the store layout, such as shelf and department layouts, product placement, lighting, music, and the like. The paper points out that the layout of the store can significantly influence purchasing behavior. For example, a well-organized and intuitive layout can increase the time spent in the store, encourage customers to research and purchase more products, and encourage impulse purchases. The importance of adjusting the layout according to the target group of customers and the type of products sold is also highlighted. Understanding and applying the principles of a well-designed layout can help sellers improve the customer experience, increase sales, and achieve a competitive advantage in the market. The research carried out in this paper aimed to investigate which elements of the store layout have the most influence on customers, which elements of the store layout customers consider the most important when purchasing or choosing a store, and how important the store layout is to customers compared to other variables of the retail mix when making a purchase or decision about the purchase. What can be concluded from this work and research is that the layout of the store is important to customers and certainly has an impact on them. More about the conducted research is explained in the last chapters of this paper.