In order for entrepreneurs to reach their full potential, it is essential to provide them with adequate support through various institutions and resources. This paper analyzes the role and functionality of the entrepreneurial support infrastructure in Osijek-Baranja County, aiming to determine how these institutions can improve the operations of small and medium-sized enterprises, which are vital to the local economy. The paper is divided into theoretical and empirical parts. The theoretical part first establishes the significance of the small and medium-sized enterprise sector in the Republic of Croatia, which is closely linked to support institutions. It then defines the entrepreneurial environment that impacts business operations, highlighting the challenges entrepreneurs face within this environment in Croatia. Finally, various types of entrepreneurial support institutions are defined to understand their operations and importance, which is further explored in the next part of the paper. In the empirical part, an analysis based on secondary data was conducted, identifying the strengths and weaknesses of entrepreneurial support in the region. It examines how different institutions can contribute to the success of entrepreneurs by providing resources, advisory services, and access to financing—key elements for the growth and sustainability of enterprises. The paper concludes with reflections on the analysis conducted and presents an overview of potential measures to enhance entrepreneurial support in Osijek-Baranja County, aiming to ensure more effective support for entrepreneurs, which could contribute to the long-term development and competitiveness of the entrepreneurial sector in the region.