This thesis defines the term environmental management system and explores the impact of its implementation in hotel enterprises with the purpose of emphasizing the importance and care of the environment as well as sustainable development, with particular focus on the example of implementing the system in the hotel company Valamar Riviera, with the help of the international standard ISO 14001. The goal of the research is to propose improvement guidelines for further environmental management within the company, based on the analyzed example of the system’s application in the selected hotel. With the use of various methods in the thesis, such as inductive and deductive methods, generalization and specialization methods, classification and description methods, and analysis and synthesis methods, and through a more detailed analysis of the implementation of the environmental management system at the level of hotel enterprises, crucial conclusions will be presented and supported with an example. The example of good practice in the company Valmar Riviera will prove that the implementation of the system has very positive effects on business operations and that it improves business processes within the company.