Organizational culture and job satisfaction are one of the most important factors that influence the efficiency and working atmosphere within an organization, shaping not only working relationships, but also the overall success of that organization. This thesis is focused on the analysis of organizational culture and its connection with job satisfaction on the example of local self-government in the Republic of Croatia. The goal of this research is to determine which organizational culture is the most represented in the Croatian public sector, more specifically in cities and municipalities that represent local self-government, and what organizational culture employees in municipalities and cities would prefer. The research also sought to determine job satisfaction in the public sector, as well as the fact that the organizational culture itself affects job satisfaction. There search was conducted through a survey questionnaire on a sample of 22 respondents, which led to the conclusion that in the cities and municipalities of the Republic of Croatia, clan organizational culture prevails, and this is also the preferred organisational culture of the respondents. While the respondents are the most satisfied with their relationship with their colleagues followed by the satisfactions with their superiors, they are the least satisfied with the possibility of advancement, which was lower in sastisfaction survey even after satisfaction with salary. The results show that there is a connection between organizational culture and job satisfaction because the characteristics of the current and preferred organizational culture are close interpersonal relationships, a sense of community and teamwork that creates a positive working environment and high trust, and such cooperation also promotes job satisfaction.