Nowdays we can often wonder how big of a role do symbols have in art generally and in cultural institutions specifically. It is necessary to know the symbols that influence the formation of an organizational culture, which is unique in every organization. Organizational culture forms the personality of the organization and its value system that it nurtures every day. In this paper, which is divided into six parts, it is explained in detail how symbols impact organizational culture of the theater and why they are important. A case study of the role of symbols and their impact on shaping the organizational culture of the Croatian National Theater and the Satirical Theater Kerempuh was conducted. Firstly, symbols are defined as elements of an organizational culture, what their origin is, their type of symbol, the importance of a symbol and their functions. After that, we specified the importance of symbols in the theater culture, what their role is and how we can manage them. After specifying the way of managing symbols in the theater, the visible and invisible symbols of the theater were analyzed through a case study for both mentioned theaters, i.e. Croatian National Theater and the Satirical Theater Kerempuh. At the end of the case study, a conclusion was given, through the interpretation, about the relationship between symbols and their importance in the organizational culture of both theaters and how the same symbols can be interpreted differently by the public.