Given the recent popularity of artificial intelligence in the scientific and business literature and speculations about the consequences of introducing artificial intelligence into organisations, the aim of this paper was to find out, through in-depth interviews from three aspects: technological, strategic and human resources, what the role of artificial intelligence in organisational structures of ambidextrous organizations is, given their proven superiority over other organisational structures. Research findings indicate that the automation will have significant impact on exploitation side of the ambidextrous structure, primarily through cost-cutting caused by the elimination of certain functions in the organisations (i.e. administration, accounting, manufacturing), while on the exploration side, artificial intelligence in the form of software and based on big data will enable creation of disruptive innovations based on insights that the human mind is unable to comprehend. To make this possible it is suggested that a central function on both sides of the structure becomes an IT function, which will integrate the implementation of new technologies, data collection and processing, and drawing insights, as well as coordinate lower levels of the organization in collaboration with the Board, while the CTO position is proposed as a responsible party for coordinating the exploitation and exploration side of the structure. In the long run, with the increasing automation and deletion of organisational functions, the gradual disappearance of formal organisations is possible.