The subject of research in this paper is the impact of the accession of the Republic of Croatia to the European Union on the operations of a logistics company. The impact is analyzed on the example of La Log d.o.o. The aim of this paper is to analyze how the accession has affected the logistics and operations of the logistics company, which conditions are changing and how Croatia and the company itself have adapted to the requirements of the European Union. The Republic of Croatia took advantage of the opportunities that arose with the accession to the European Union, which is evident from the business results of logistics companies. International trade in goods has increased, as have the turnover and revenues of logistics companies. All of the above also contributed to a significant increase in employment in this sector. In 2019, a total of 13.527.194 thousand tons of goods were transported by road in the European Union (EU-27). The share of Croatia is small and amounts to only 0,60%, but there is a growth trend. At the level of the Republic of Croatia, the largest three entities in the activity of transport of goods hold a 5,51% share in operating revenues, and the largest ten 13,23%. La Log d.o.o. is in its activity according to the realized revenues on the 3rd place with a share of 1,58%. La Log's financial indicators indicate successful business even before Croatia's accession to the European Union, but most financial indicators record better results after joining the country in the Union.