Autism spectrum disorder is a complex neurodevelopmental disorder that affects all aspects of an individual's life and, consequently, the individual's family. It has been proven that parents of children with autism spectrum disorder experience higher levels of stress than parents of typically developing children, but also parents of children with other developmental disabilities. Precisely for this reason, this research aims to examine the differences in the stress sources and intensity of mothers and fathers of children with autism spectrum disorder. 15 mothers and 15 fathers from 15 families participated in the research. Parents filled out the Parenting Stress Index, Fourth Edition Short Form, and the Sociodemographic questionnaire created to conduct the research. In this research, no statistically significant differences were found in the total amount of stress between mothers and fathers, but it was determined that 10 parents (33%) achieve a significant amount of stress. The overall level of stress in both mothers and fathers is most affected by the demands of the child. The causes of stress in mothers are more often the child's emotional problems and withdrawal, while in fathers the causes of stress are externalizing behaviors of the child. The severity of symptoms in the child was shown to be related to the overall level of stress in parents. The more severe the symptoms, the higher the stress levels parents experience. The association between socioeconomic factors and the total level of stress in parents was not found. Research on this topic is essential for more successful provision of help and support to parents of children with autism spectrum disorder.