The aim of this work was to examine the presence of internalizing symptoms in adolescents who consume alcohol and marijuana. The aim was also to establish the prevalence of symptoms of anxiety and depression, as well as the prevalence of alcohol and marijuana consumption. In addition, the differences in the relationship between symptoms of anxiety and depression with the consumption of alcohol and marijuana were examined with regard to the gender of adolescents. The research was carried out as part of a larger scientific project "Testing the 5c model of positive youth development: traditional and digital mobile measurement (P.R.O.T.E.C.T.) (UIP - 2020 - 02 - 2852)" which is carried out by the Faculty of Education and Rehabilitation in Zagreb. For the purposes of this paper, a sample of 326 subjects of the adolescent population was selected from the second point of measurement between the ages of 15 and 17, and for the purpose of examining the target constructs, the Depression, Anxiety and Stress Scale-21 (Lovibond and Lovibond, 1995) and part of the questionnaire The Alcohol, Smoking and Substance Involvement Test (WHO, 2010). The obtained data were processed using methods of descriptive statistics and non-parametric correlations in the computer program SPSS Statistics 29.0. The research results show that in more than half (60.7%) of the respondents, anxiety symptomatology is present to an insignificant extent, and serious and very serious symptomatology is present in 9.2% of the participants. Depressive symptoms are reported by 71.8% of respondents to an insignificant extent, while depression belongs to the most risky group of severe and extremely severe symptomatology in 1.5% of respondents. The lifetime prevalence of drinking more than a few sips of alcoholic beverages is 77.3%, while the prevalence of marijuana consumption is 16.30%. A statistically significant negative correlation was found between certain aspects of alcohol and marijuana consumption and symptoms of anxiety and depression, and more statistically significant correlations were found between symptoms of anxiety and depression and alcohol and marijuana consumption in the female gender than in young men.