The aim of this research paper is to examine the voice quality of theater actors and determine the link between self-assessment and objective vocal evaluation. A total of 37 educated actors from the city of Zagreb participating in the study were subject to self-assessment, which comprised the Voice Handicap Index (VHI) and a specialized Vocal Self-assessment Questionnaire for Actors, and to objective evaluation, which included acoustic and phonation analyses of the sound /a/ using the PRAAT Program. The observed acoustic variables consisted of jitter, shimmer and the harmonics-to-noise ratio (HNR). Phonations of the sound /a/ were recorded using a TASCAM DR-05. A statistical analysis showed a statistical positive correlation between overall score on F subscale of the VHI and the observed acoustic variable HNR and a statistical correlation between the Vocal Self-assessment Questionnaire for Actors and the observed acoustic variables of jitter (negative correlation) and HNR (positive correlation). Moreover, a statistical correlation was established between the Voice Handicap Index and the Vocal Self-assessment Questionnaire for Actors. The ensuing results speak in favor of existing research which emphasize the need for an independent interpretation of subjective and objective vocal assessment methods. Furthermore, the issue was raised about the further development and psychometric testing of the Vocal Self-assessment Questionnaire for Actors to turn it into a measuring instrument intended exclusively for this population of elite vocal professionals.