The aim of this study was to perform a quantitative analysis of socio-emotional difficulties and difficulties in performing daily activities that may result from tinnitus in adults and the elderly, and to examine their possible correlation with the demographic characteristics of research participants and their audiological characteristics. The research included translation, adaptation and psychometric verification of the Tinnitus Handicap Inventory (THI) questionnaire, since an instrument like that does not exist in Croatia. The study involved 99 patients from a clinical hospital in Zagreb who came for an audiometric hearing test at the general otolaryngology outpatient department of the Otorhinolaryngology and Head and Neck Surgery, who indicated that they had tinnitus during an audiometric examination. The Croatian adaptation of the THI questionnaire showed excellent reliability (Cronbach's alpha of 0.95) and a high degree of repeatability of the results (confirmed by Intraclass correlation coefficient). The validity of the questionnaire was examined by analysing the significance of Spearman's correlation coefficients between self-reported loudness and tinnitus discomfort and the overall score of the THI-H questionnaire and its subscales. The Spearmen correlation between the total score of the THI-H questionnaire and the results of its subscales with the results of the subscales of the Croatian version of the Depression Anxiety and Stress Scales (DASS-21) questionnaire was also analysed. The results showed a low-to-moderate significant positive correlation of perceived volume and tinnitus discomfort as well as the DASS-21 questionnaire subscales with the overall scores of the THI-H questionnaire and its subscales. The correlation of tinnitus with demographic and audiological data has been shown to be low and practically insignificant. Considering the results of the research, the Croatian adaptation of the THI questionnaire is reliable and valid and can be used for clinical purposes. Furthermore, the findings demonstrate the complexity of the impact of tinnitus on daily life and encourage a multidisciplinary approach to supporting people with tinnitus