Sign language is a natural communication system of the Deaf community that has all components of human language and grammar rules that are completely independent of the spoken language. Due to the need for introducing sign language in family centered early intervention and (re)habilitation programs for deaf and hard of hearing children, there is a growing need for a systematic and comprehensive description of Croatian Sign Language (HZJ) grammar. The aim of this study was to determine the role of eyebrow position in HZJ grammar, and to determine whether it differs from the eyebrow position in Croatian spoken language (HJ). Video materials used in this research were a part of the database previously collected for the purpouse of HZJ research (corpus), and video materials collected for comparison with HJ. For data analysis, a specialized program for linguistic analysis ELAN and Microsoft Office Excel program were used. Data were processed by descriptive analysis. The results have showen that the position of the eyebrows have an important role in the grammar of HZJ, in distinguishing various types of sentences, such as negative, conditional and interrogative sentences, then topics, focus and contrasting focus in the sentence, intensification, but also has a role in pragmatics, and prosody. The results are comparable to research in other sign languages, and contribute to the description of HZJ grammar, which will promote the development of family centered early intervention programs, and the development of relationship and communication between deaf children and their hearing parents, as well as other members of the child's environment.