Introduction: Intravenous thrombolysis is the only approved systemic reperfusion treatment for patients with acute ischemic stroke. Intravenous thrombolys is remains the cornerstone of the treatment of acute stroke. Appropriate patient selection and timely treatment are crucial. Objective: to examine students' attitudes towards the treatment of acute stroke by thrombolysis and compare them according to socio-demographic characteristics. Methods: online questionnaire Results: Students' attitudes towards the treatment of acute stroke are positive. A significant difference was found according to gender, with men having a more positive opinion on the use of thrombolytic therapy. Significant difference according to respondents’ age was shown in the statement "Thrombolysis only further endangers the life of the patient", where the respondents aged 36 and over showed a significantly more negative opinion compared tothe respondents aged 18-25 (P = 0.001) and respondents aged 26-35 years (P = 0.4); in the statement “Would you agree to a thrombolysis procedure for yourself” the respondents aged 18-25 showed a significantly more positive opinion than the respondents aged 36 and over (P = 0.02) and respondents aged 26-35 (P = 0.02); and in the statement "Would you agree to a thrombolysis procedure for your loved ones", where the respondents aged 18-25 showed a significant negative opinion. Conclusion: After processing the results and comparing them with other studies, it can be concluded that respondents are mostly aware of the importance of treating acute stroke with thrombolysis and attitudes towards thrombolysis are positive, but confidence in the application of thrombolysis on themselves or their loved ones is not great. Therefore, education and training about its importance are necessary, especially among students, who are future highly educated health professionals.