The aim of this study was to examine the effects of interaction between age and physical activity on quality of life. The study included 182 respondents employed in several institutions and companies in Osijek. Measurement of a Person's Habitual Physical Activity (Baecke et al, 1982) and Personal wellbeing index (Personal Wellbeing Index – PWI; International Wellbeing group, 2013) were used. The results show that the main effect of gender does not explain quality of life while the main effect of physical activity was significant. The results also show that the interaction between age and physical activity does not explain a quality of life. When examining the relationship between different areas of physical activity and quality of life, a positive correlation between quality of life and physical activity in sport and physical activity in leisure time was found, while there was no significant correlation between quality of life and physical activity at work. When examining the relationship between age and different areas of physical activity, a positive correlation between age and physical activity at work and negative correlation between age and physical activity in sport was found. No significant correlation has been found between age and physical activity during leisure time. The investigation whether age and gender contribute to overall physical activity have shown that the main effects of gender and age were not significant. The interaction effect of age and gender also was not significant.