The aim of this research was to compare some aspects of the psychological well-being of recreational runners and people who are not regularly physically active. 717 participants between the ages of 18 and 65 took part in the research. Total of 252 participants belonged to the group that did not engage in any kind of physical activity, and 465 participants represented the group of recreational runners. The research was conducted online, and the Sociodemographic Data Questionnaire, the Questionnaire on physical activity, the Questionnaire on running, the Depression Anxiety Stress Scales (DASS), Brief Resilience Scale (BRS) were used. The results indicated statistically significantly lower levels of depression, anxiety and stress among recreational runners, compared to the participants who were not engaged in any kind of physical activity. In addition, characteristics of running training were analyzed. Although the correlations between levels of depression, anxiety, and stress and the average number of kilometers run per week were negative, they did not reach statistical significance. Furthermore, groups did not differ in psychological resistance, nor was runner's resistance significantly related to the average number of kilometers run per week, the number of training sessions per week, and the length of training in years. This research, in this area, is one of the first to compare the important aspects of psychological well-being of two different groups and to connect the characteristics of running training with the mentioned aspects.