Starting from the educational role of the museum, the central theme of this paper is - collaboration between museums and high schools. The aim of the paper is to determine the current state and level of collaboration between museums and high schools and create the preconditions for the development of guidelines for enriching the cooperative relations between these two institutions. The first part of the paper presents the benefits arising from mutual collaboration, problems and obstacles, conditions for successful collaboration and specific models of collaboration. The second part of the paper presents an empirical study using quantitative methodology where the data were collected using an online survey, in which 256 high school teachers participated. The results of the research showed that teachers of gymnasiums, vocational and art schools consider visiting a museum and the content it offers useful and necessary in the high school system, while a higher rate of museum visits was recorded by teachers of gymnasium programs. The provider-receiver model of collaboration stands out as the most common model of collaboration, in which the dominant role in the implementation of cooperative relations is played by the museum institution, while the high school is the passive recipient of services. Highlighting specific barriers to establishing cooperation, such as lack of time, interest and appropriate programs, most concluded that they saw the potential and wanted to collaborate with museums in the long run, giving some guidelines on how to improve collaboration