Electrospinning is process of nanofiber production using electrostatic forces. Human tissue is prone to damage. Smaller wounds, scratches and sprains, and even milder fractures of the bones are easily healed. The problem are completely damaged organs that lose function as well as large-scale bone fractures. In addressing this problem, tissue engineering has a solution. Tissue engineering and manufacturing of functional tissues and organs are part of modern biomedical research and application. In tissue engineering cells are planted in biomaterials prior to transplantation. These materials then serve as temporary carriers that improve cell reorganization to form functional tissue. The carriers are made of biocompatible and or biodegradable materials. Recently, biodegradable fiber polymers are used as matrices for the cultivation and growth of different tissue cells. Specifically, scaffolds obtained by electrospinning of poly (ε-caprolactone) (PCL) exhibit good potential as skin regeneration tissue carriers. In this work by electrospinning procedure scaffolding materials were produced: polycaprolactone (PCL) scaffold (100% PCL), combined bi-polymer scaffold silk fibroin (SF) (18% PCL+50% FS), (PCL) scaffold with antibiotic Cefuroxim (18 % PCL+25 % CFU) and 18% PCL+50% SF with incorporation of 0,5 mas.% of micro and nano TiO2. Morphology, viscoelastic properties and hydrofobicity of the scaffolds were characterized to evaluate the effect of FS,CFU and TiO2 by means of scanning electron microscopy (SEM), dynamic mechanical analysis and contact angle.