Chitosan is linear, semi-crystalline polysaccharide composed of N-acetyl D-glucosamine and D-glucosamine units. It can be easily derived from partial deacetylation of natural polymer chitin. Thanks to the amino groups of D-glucosamine, chitosan can be protonated and turned into polycation, which is one of sources of unique properties of chitosan as biopolymer, like aqueous solubility, antibacterial properties, biodegradability with non-toxic residues and biocompatibility. Chitosan is only known positively charged polysaccharide. Thanks to the strucure and chemical components, chitosan can form 3-D scaffolds, in form of hydrogels and sponges, which can replace damadged tissue or organ, and allows interaction with surrounding tissue cells and proliferation. Three types of hydrogels have been developed, presenting either reversilbe or irreversible gelation. Chitosan can be physically associated, coordinated with metal ions or irreversibly/ chemically cross- linked into hydrogels. Chitosan sponges are used as wound healing materials, as they can soak up the wound exudated, while helping the tissue regeneration. These sponges are mainly obtained by lyophilization, a simple efficient process consisting in freezing a solution of chitosan followed by sublimation of solvent under reduced pressure. In some cases when it is not suitable to use 3-D scaffolds, 2-D scaffolds are used: thin films and porous nanofiber membranes. Two methods to design chitosan thin films are developed: Langmuir-Blodgett, in which a solid support is immersed in aqueous phase covered in polymer monolayer, and the layer-by-layer deposition technique, based on the lternated adsorption of materials bearing charged or functional groups in aquous medium. For chitosan nanofiber production elecrospinning method is used, in which polymer solution in injected onto a plate through high-voltage electric field, which formes fibers.