Enzymes are proteins whose primary task is to catalyse biochemical reactions, where each is catalyzed by a unique enzyme. Proteases are one of the most important groups of enzymes, and their application in industry is growing daily. The reason for this is economic profitability due to low production costs and low energy consumption, high yields and simplicity of genetic modifications. In this paper, the efficiency of enzymes in the hydrolysis of proteins from shrimp waste was examined using six commercial proteases: Alcalase, Neutrase, Esperase, Flavourzyme, Novozyme and Novo-ProD. All enzymes were successful in protein degradation. It was found that by performing hydrolysis even at lower enzyme concentrations, the conversions are almost equally high in most cases, except by Neutrase where the conversion is a bit lower. For most enzymes, high conversions were achieved in the first hour of experiment, which was not the case with Neutrase as the conversion gradually increased. Upon completion of protein hydrolysis, Alcalase, Flavourzyme, and Novo-ProD showed the highest stability at an enzyme concentration of 0.5 U / 10 mg, and were therefore selected to catalyse protein hydrolysis in a repetitive reactor with six cycles. Alcalase and Novo-ProD showed satisfactory results in terms of high conversions and activity after the last cycle.