Analytical method validation is the first step towards ensuring the reliability and accuracy of analytical results. Validation verifies that our method is suitable for its intended purpose and also enables method accreditation. It is a regulatory requirement as well as the professional responsibility of an analyst. To validate an analytical method, it is necessary to evaluate all the method parameters: specificity/selectivity, linearity, precision, trueness, accuracy, detection limit, quantification limit, working range and robustness. The aim of this study was to validate the spectrophotometric method for the determination of the pharmaceutical ciprofloxacin, to get acquainted with the validation procedure and based on the obtained results to conclude whether the suggested method meets te acceptability criteria. Solutions of ciprofloxacin in Millipore water were prepared, and all validation parameters were examined. The linearity of the method was confirmed in the concentration working range from 4,60 mg/L to 30 mg/L with the coefficient of determination R^2=0,9984. The quantification limit is at a concentration of 4,60 mg/L, and the detection limit is at a concentration of 1,52 mg/L. The results of this study showed that the method is acceptable for the determination of ciprofloxacin in the defined working range within which all validation parameters meet predetermined acceptability criteria.