Optically pure compounds are of great significance in the pharmaceutical industry and can be produced by enantioselective biocatalysis whose two methods are kinetic resolution and asymmetric synthesis. The conversion of the initial compounds to optically pure products by kinetic resolution does not exceed 50 %. This drawback is solved by dynamic kinetic resolution (DKR) which is based on kinetic resolution combined with in situ racemization of slower reacting enantiomer. Under adequate conditions, a racemic mixture can be transformed into one enantiomer by dynamic kinetic resolution. DKR efficiency depends on the structural parameters of substrates and catalysts as well as the conditions of in situ racemization. The mathematical model consists of the kinetic and the reactor model. In this paper, validation of the mathematical model of dynamic kinetic resolution for obtaining (S)-5-bromomethyl-5-methyloxazolidin-2-one from rac-2-bromomethyl-2-methyl-oxirane catalyzed by HheC enzyme extracted from Agrobacterium radiobacter AD1 is conducted. The model is divided into three reaction steps, where the first one is the reaction of (S)-epoxide and cyanate ions with the formation of (S)-oxazolidinone. Simultaneously, the reaction with (R)-epoxide and cyanate ions forming (R)-oxazolidinone is happening. Furthermore, the second step is the formation of dibromoalcohol from (R)-epoxide and bromide ions as well as (S)-epoxide and bromide ions, while the third step is the formation of rac-epoxide from dibromoalcohol. HheC used in the experiment is one of the few structurally characterized halohydrin-dehalogenases. The kinetic of this reaction was determined in previous papers. While conducting validation experiments in the batch reactor it was recorded that these enzymes have a different enantiomeric affinity. Experimental data have shown a satisfactory match with the earlier developed model. Moreover, optimal conditions for dynamic kinetic resolution for obtaining optically pure (S)-5-bromomethyl-5-methyloxazolidin-2-one were stipulated.